| Authors |
Baranova Irina Petrovna, Doctor of medical sciences, professor, head of sub-department of infectious diseases, Penza Institute of Advanced Medical Studies – branch of Russian Medical Academy of Lifelong Vocational Training (8a Stasova street, Penza, Russia), irinapetrovna.baranova@yandex.ru
Malova Irina Anatol'evna, Postgraduate student, Penza Institute of Advanced Medical Studies (8a Stasova street, Penza, Russia); head of infectious diseases unit, Medical and Sanitary Unit №59 of the Federal Biomedical Agency of Russia (8 Sportivnaya street, Zarechny, Penza region, Russia), infekz@msch59.ru
Baranova Nadezhda Ivanovna, Doctor of medical sciences, associate professor, assistant, sub-department of allergology and immunology, Penza Institute of Advanced Medical Studies – branch of Russian Medical Academy of Lifelong Vocational Training (8a Stasova street, Penza, Russia), infekz@msch59.ru
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| Abstract |
Background. The authors examined indicators of spontaneous and induced γ-interferon in hospitalized patients, depending on the age of patients, gender and the etiology of the disease.
Materials and methods. The researchers conducted a study of patients treated in the infectious department of the Medical and Sanitary Unit No. 59 of the Federal medical-biological Agency of Russia in the town of Zarechny, Penza region in the period 2014–2016. In 64 patients the SARS and influenza etiology was verified by detection of RNA and DNA viruses in the nasopharyngeal secretion using the polymerase chain reaction method in real time. The material samples were taken during the first days of patients’ stay in the hospital. Swabs were taken from both nasal passages by dry cotton wicks and were subsequently placed in test tubes with 0.5–1.0 ml. of medium. To verify SARS causative agents in the clinical material the authors used the polymerase chain reaction (PCR) with hybridization-fluorescent detection by a reagent set "AmpliSensORVI-screen-FL". The amplification reaction was performed using a "DT-96" PCR device («DNA-technology», Russia) in "real time". The study was conducted on the basis of the Medical and Sanitary Unit No. 59 of the Federal medical-biological Agency of Russia. The patients were subject to examination of their spontaneous and induced γ-interferon by the method of immune- enzyme analysis using a set of reagents "gamma-interferon-Elisa-best" (CJSC "Vector-best", Novosibirsk, Russia). A statistical analysis was performed using Statistica program 10 Rus (StatSoft Inc. USA). The original qualitative and quantitative indicators were compared using the student's t-test and the Fisher’s criterion of least significant difference; the reliability of the "p" indicator when comparing the signs in small groups of patients were determined by the V.S. Gennes’ method.
Results. The PCR method verified activators of influenza A and B, RS viral infection among 64 patients approximately in equal proportions; in 23 % of cases the pathogen was not verified. The test group was dominated by children: 43 children aged from 3 months to 14 years, which accounted for 67 % of the sample, against 21 (33 %) adults. There were found no statistically significant gender differences among patients. There were revealed no significant differences in indicators of the level of spontaneous γ-interferon before and after treatment and induced γ-interferon before and after treatment in the test group, but there was a significant difference between the rates of spontaneous and induced γ-interferon, both before and after treatment. The level of induced γ-interferon, defined before the treatment, significantly fluctuated depending on viral infection nosology. Statistically significant differences in the average level of spontaneous and induced γ-interferon both before and after treatment among patients, males and females, were identified. In patients older than 6 years the level of induced γ-interferon was significantly higher than in preschool children.
Conclusions. 1. The study confirmed that indicators of the level of induced γ-interferon are hundreds of times higher than ones of the level of spontaneous γ-interferon. 2. There were revealed no significant differences in terms of the level of spontaneous γ-interferon before and after treatment and induced γ-interferon before and after treatment in a group of subjects. 3. The level of spontaneous γ- interferon before and after treatment was low and ranged 0.0–4.9 pg/mL, without any dependence on the etiology, age and gender. 4. The indicators of induced γ- interferon as a whole depended on the etiology of the causative agent, the patients’ age, but not gender. 5. The level of induced γ-interferon before treatment was significantly higher in conditions of influenza than at respiratory-sincitial infections. 6. The level of induced γ-interferon before and after treatment was significantly higher among children of school age and adults than in preschool children.
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